产品介绍
FAM hydrazide, 5-isomer
Many natural and synthetic molecules contain aldehyde or ketone carbonyl groups. These carbonyls react with hydrazides with the formation of hydrazones. The reaction is spontaneous at pH values around neutral, and the resulting hydrazones are very stable.
pounds containing 1,2-diol function, like sugars, can be oxidized with sodium periodate with the formation of carbonyl pounds for the subseuent modification with hydrazides. This is an efficient method for the labeling of glycoproteins (like antibodies), and polysaccharides.
FAM hydrazide is a hydrazide label for the attachment of fluorescein, a bright dye for 488 nm channel, to carbonyl pounds.
General properties
Appearance:
yellow solid
Mass spec M+ increment:
372.07
Molecular weight:
426.81
Molecular formula:
C21H15N2ClO6
Solubility:
good in ethanol, DMF, DMSO
uality cont*ol:
NMR 1H, HPLC-MS (95%)
Storage conditions:
Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
Spectral properties
Excitation maximum, nm:
494
ε, L⋅mol−1⋅cm−1:
75000
Emission maximum, nm:
520
Fluorescence uantum yield:
0.9
CF260:
0.20
CF280:
0.17
FAM hydrazide,酰肼绿色荧光染料
西安NG体育科技有限公司
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