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157843-41-9, 2-Chloro-4-nitrophenyl α-L-fucopyranoside, CAS:157843-41-9 C12H14ClNO7 / 319.69 MFCD049720562-Chloro-4-nitrophenyl a-L-fucopyranoside CAS No: 157843-41-9 MDL No: MFCD04972056 Chemical Formula: C12H14ClNO7 Molecular Weight: 319.69 Product Structure COA: Name: 2-Chloro-4-nitrophenyl-alpha-L-fucopyranoside; CNP-AFU; CNPF CAS: 157843-41-9 M.F.: C12H14ClNO7 M.W.: 319.69 Items Standards Results Appearance White crystal powder Complies Solubility Soluble in DMF, insoluble in petroleum ether Complies Identification IR and HPLC Complies MS and NMR Should comply Complies Melting point 150℃ ~  160 °C 155℃ ~158 ℃ Specific rotation ( [α]20/D) -163o ~  -173o -172.5o Any other impurity Max. 0.5% Complies Heavy metal Max. 20ppm Complies Residue on ignition Max. 0.5% 0.1% TLC Should be one spot One spot Assay by HPLC Min. 98% 99.2% Chromogenic substrate for alpha-L-Fucosidase. The AFU assay kit is based on the enzymatic cleavage of the synthetic substrate CNPF to α-L-fucose and 2-chloro-4-nitrophenol, which is quantified by measuring the absorbances at 405 nm in a kinetic fashion. It is a one step assay with a single assay reagent. One unit of AFU is defined as the amount of AFU that cleaves one μmole of 2-chloro-4-nitrophenyl-α-L-fucoside per min at37 °C.