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FAM tetrazine,5-isomer,荧光素-四嗪 Tetrazine (methyltetrazine) derivative of fluorescein, pure 5-isomer. Tetrazines are reactive heterodiene components for [4+2] inverse electron demand Diels-Alder reaction (ied-DA) with strained cycloalkenes (trans-cyclooctenes, cyclopropenes). This reaction is also called TCO (tetrazine-cyclooctene) ligation. The reaction is among the fastest bioconjugation reactions. General properties Appearance: yellow solid Mass spec M+ increment: 529.12 Molecular weight: 559.53 Molecular formula: C31H21N5O6 Solubility: good in DMF, DMSO Quality control: NMR 1H, HPLC-MS (95%) Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. Spectral properties Excitation maximum, nm: 490 ε, L⋅mol−1⋅cm−1: 80000 Emission maximum, nm: 513 Fluorescence quantum yield: 0.93 CF260: 0.20 CF280: 0.17 FAM tetrazine,5-isomer,荧光素-四嗪 叠氮化物 Dye azides Pyrene azide 1/ Pyrene azide 2 PEP azide Perylene azide BDP FL azide Coumarin 343 azide FAM azide, 5-isomer FAM azide, 6-isomer TAMRA azide, 5- isomer BDP TMR azide Cyanine3 azide Cyanine3.5 azide Cyanine5 azide Cyanine5.5 azide Cyanine7 azide Cyanine7.5 azide Sulfo-Cyanine3 azide Sulfo-Cyanine5 azide Sulfo-Cyanine7 azide N-羟基琥珀酰亚胺脂Dye NHS esters Pyrenebutyric acid NHS ester ROX NHS ester, pure 6- isomer BDP FL NHS ester FAM NHS ester Cyanine3 NHS ester Cyanine3.5 NHS ester Cyanine5 NHS ester Cyanine5.5 NHS ester Cyanine7 NHS ester Cyanine7.5 NHS ester Sulfo-Cyanine3 NHS ester Sulfo-Cyanine5 NHS ester sulfo-Cyanine5 bis-NHS ester Sulfo-Cyanine7 NHS ester