Sulfo-Cyanine5 NHS ester|146368-14-1
水溶Cyanine 5琥珀酰亚胺酯(succinimidyl ester(SE)),类似于Cy5® NHS ester,不需要有机溶剂即在水相中即可对各种带氨基分子进行标记。因此该产品尤其适用于对那些在有机溶剂中易变性的蛋白和低溶解度蛋白的标记。
Sulfo-Cyanine 5是Cy5®的衍生物,与很多仪器如读板机(plate readers),显微镜和成像设备等相兼容。是的荧光染料之一。
该染料是高度亲水的、水溶的。非磺化的衍生物:Cyanine 5 NHS ester。
可以在所有的应用中替代Cy5®,Alexa Fluor 647和DyLight 649。
Sulfo-Cyanine5 NHS ester|146368-14-1
一般特性
外观:
dark blue powder
分子量:
761.84
分子式:
C36H40N3NaO10S2
溶解性:
very good in water, good in DMF and DMSO
质量控制:
NMR 1H (95%) and 13C, TLC, functional testing
储存条件:
Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
光谱特性
zui大激发波长, nm:
646
在zui大激发波长下的消光系数, Lmol-1cm-1:
271000
zui大发生波长, nm:
662
荧光量子产率:
0.2
CF260:
0.13
CF280:
0.13
Cy系列的活性基团菁染料:
N-羟基琥珀酰亚胺脂Dye NHS esters
Pyrenebutyric acid NHS ester
ROX NHS ester, pure 6- isomer
BDP FL NHS ester
FAM NHS ester
Cyanine3 NHS ester
Cyanine3.5 NHS ester
Cyanine5 NHS ester
Cyanine5.5 NHS ester
Cyanine7 NHS ester
Cyanine7.5 NHS ester
Sulfo-Cyanine3 NHS ester
Sulfo-Cyanine5 NHS ester
sulfo-Cyanine5 bis-NHS ester
Sulfo-Cyanine7 NHS ester
马来酰亚胺 Dye maleimides
Pyrene maleimide
BDP FL maleimide
Cyanine3 maleimide
Cyanine5 maleimide
Cyanine5.5 maleimide
Cyanine7 maleimide
Cyanine7.5 maleimide
Sulfo-Cyanine3 maleimide
Sulfo-Cyanine5 maleimide
伯胺 Primary amine
BDP FL amine
Cyanine3 amine
Cyanine5 amine
Cyanine5.5 amine
Cyanine7 amine
Cyanine7.5 amine
Sulfo-Cyanine5 amine
Sulfo-Cyanine7 amine
酰肼 Dye hydrazides
Pyrene hydrazide
BDP FL hydrazide
Cyanine3 hydrazide
Cyanine5 Boc-hydrazide
Cyanine5 hydrazide
Cyanine5.5 hydrazide
Cyanine7 hydrazide
Cyanine7.5 hydrazide
马来酰亚胺 Dye maleimides
BDP FL maleimide
Cyanine3 maleimide
Cyanine5 maleimide
Cyanine5.5 maleimide
Cyanine7 maleimide
Cyanine7.5 maleimide
Sulfo-Cyanine3 maleimide
Sulfo-Cyanine5 maleimide
羧酸 Carboxylic acids
Pyrenebutyric acid
BDP FL carboxylic acid
Cyanine3 carboxylic acid
Cyanine3.5 carboxylic acid
Cyanine5 carboxylic acid
Cyanine5.5 carboxylic acid
Cyanine7 carboxylic acid
Cyanine7.5 carboxylic acid
Sulfo-Cyanine3 carboxylic acid
Sulfo-Cyanine5 carboxylic acid
Sulfo-Cyanine7 carboxylic acid
点击化学试剂
非荧光炔烃 Non-fluorescent alkynes
Pentynoic acid STP ester
Biotin alkyne
非荧光叠氮化物 Non-fluorescent azides
Azidobutyric acid NHS ester
Biotin azide
寡核苷酸分析
亚磷酰胺 Phosphoramidites
FAM Phosphoramidite, 6-Isomer
Alkyne Amidite, hydroxyprolinol
Alkyne Phosphoramidite, 5'-terminal
DMS(O)MT aminolink C6
Long trebler phosphoramidite
Pyrene phosphoramidite dU
PCR/PCR PCR/PCR
ROX reference dye for PCR
SYBR Green I Gel Staining Solution, 10000x
SYBR Green I for Real Time PCR, 100x
CPG固相修饰 CPG solid supports
Alkyne CPG modifier 500
TFA-Amino modifier CPG 500
三磷酸酯修饰 Modified triphosphates
Ami1-ddUTP
Ami1-dUTP