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Cy5 Picolyl Azide|Cyanine5 Picolyl Azide
   
Cy5 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site. The chelating effect of picolyl azide moiety to raise the effective concentration of copper at the reaction site is so great that it leads to an increase of signal intensity of up to 40-fold.
In addition, the use of Cy5 Picolyl Azide instead of conventional Cy5-Azide allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling.
In summary, the introduction of a picolyl moiety into an azide probe leads to a substantial increase in the sensitivity of alkyne detection. This will be of special value for the detection of low abundance targets or where significant increase in signal intensity is desired.
structural formula：
    
Cy5 Picolyl Azide|Cyanine5 Picolyl Azide
Abs/Em Maxima
647/663 nm
Extinction coefficient
250,000
Flow cytometry laser line
633 or 635 nm
Microscopy laser line
633 or 635 nm
Spectrally similar dyes
Alexa Fluor® 647, Atto™ 647, CF™ 647 Dye, DyLight™649
Molecular weight
953.15
CAS
N/A
Solubility
Water, DMSO, DMF, MeOH
Purity
>95% (HPLC)
Appearance
Blue solid
Storage conditions
-20C
Shipping conditions
Ambient temperature
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TAMRA alkyne, 5-isomer
 
Sulfo-Cyanine7 alkyne
 
Sulfo-Cyanine5.5 alkyne
 
磺酸基-Cy5 炔烃 Sulfo-Cyanine5 alkyne
 
Sulfo-Cyanine3 alkyne
 
ROX alkyne, 5-isomer
 
FAM alkyne, 6-isomer
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Cy5.5 炔烃 Cyanine5.5 alkyne
 
Cyanine5 alkyne
 
Cyanine3 alkyne
 
BDP TMR alkyne
 
BDP FL alkyne
 
BDP 630/650 alkyne
 
3-Ethynyl perylene
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1-Ethynyl pyrene
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Sulfo-Cyanine7 azide
 
Sulfo-Cyanine5.5 azide
 
Sulfo-Cyanine5 azide
 
Sulfo-Cyanine3 azide
 
ROX azide, 5- isomer
 
R110 azide, 6- isomer
 
R110 azide, 5- isomer
 
Pyrene azide 3
 
Pyrene azide 2
 
Pyrene azide 1
 
Perylene azide
 
PEP azide
 
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BDP TR methyl ester, 5 mM in DMSO
 
Cyanine5 DBCO
 
Sulfo-Cyanine5 tetrazine
 
Sulfo-Cyanine3 tetrazine
 
TAMRA amine, 5-isomer
 
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Sulfo-Cyanine5.5 amine
 
   
Sulfo-Cyanine3 amine
 
FAM amine, 5-isomer
 
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Cy7 胺 Cyanine7 amine
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Cy5 胺 Cyanine5 amine
 
Cyanine3 amine
 
BDP TMR amine
 
BDP FL amine
 
BDP 630/650 amine