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6-TAMRA Azide|6-TAMRA N3
6-TAMRA Azide (also known as TAMRA azide, isomer 6) is the red-fluorescent probe that is patible with various excitation sources including mercury arc, tungsten and xenon arc lamps, the 544 nm line of the Helium-Neon laser and the 532 nm green laser line. It is predominantly used for detection of terminal alkyne-tagged biomolecules via a copper-catalyzed click reaction (CUAAC). It also reacts with strained cyclooctyne via a copper-free &lduo;click chemistry&rduo; reaction to form a stable triazole and does not reuire Cu-catalyst or elevated temperatures.
Although the mixed isomers of 5(6)-TAMRA azide is a preferred, routinely used red fluorescent dye for staining proteins, labeling peptides and nucleotides with TAMRA mixed isomers might be troublesome due to significant signal broadening in HPLC purification. Peptides and nucleotides labeled with a single isomer TAMRA usually give better resolution in HPLC purification that is often reuired in the conjugation processes.
   
6-TAMRA Azide|6-TAMRA N3
Abs/Em Maxima
553/575
Extinction coefficient
89,000
Flow cytometry laser line
532 or 555 nm
Microscopy laser line
532 or 555 nm
Spectrally similar dyes
Alexa Fluor® 546, Atto™ 543, CF™ 555 Dye, DyLight™549
Molecular weight
555.67
CAS
n/a
Solubility
Water, DMSO, DMF, MeOH
Purity
>98% (HPLC)
Appearance
Red solid
Storage conditions
-20C
Shipping conditions
Ambient temperature
叠氮化合物(Azides)
Azidobutyric acid NHS ester
Azido dyes, available as solids, and as ready-to-use 10 mM DMSO solutions
Water-soluble Cy3 azide
Water-soluble Cy5 azide
Cy3 azide
Cy3.5 azide
Cy5 azide
Cy5.5 azide
Cy7 azide
Cy7.5 azide
5-FAM azide
6-FAM azide
5-JOE azide
5-R110 azide
6-R110 azide
6-ROX azide
5-TAMRA azide
炔烃类染料(Alkynes)
Alkyne phosphoramidite, 5'-terminal
Alkyne amidite, hydroxyprolinol
Pentynoic acid STP ester
荧光激活染料(Catalysts and activators)
Ascorbic acid
Copper(II)-TBTA plex, 10 mM in 55% a. DMSO
SYBR Green I
Gel staining solution, 10000x
For PCR, 100x solution
改良三磷酸盐(Modified triphosphates)
Ami1-ddUTP
Ami1-dUTP
脂类活性染料(Dye activated esters)
Cy3 NHS ester (water soluble)
Cy5 NHS ester (water soluble)
Cy3 NHS ester
Cy3.5 NHS ester
Cy5 NHS ester
Cy5.5 NHS ester
Cy7 NHS ester
Cy7.5 NHS ester
ROX NHS ester, pure 6- isomer
羰基活性染料(Dye hydrazides)
Cy5 hydrazide
巯基反应性染料(Dye maleimides)
Cy5 maleimide
Cy5.5 maleimide
2D DIGE荧光染料试剂盒(2D DIGE dye kits)
Phosporamidites
Alkyne phosphoramidite, 5'-terminal
Alkyne amidite, hydroxyprolinol
DMS(O)MT aminolink amidite
Long trebler amidite
Pyrene dU amidite